4L60

Structure of C81R Mutant PCNA Protein Defective in Mismatch Repair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-03
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 21 3
Unit cell lengths	121.822, 121.822, 121.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.500 - 3.003
R-factor	0.2011
Rwork	0.198
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
RMSD bond length	0.013
RMSD bond angle	1.573
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.500	4.754
High resolution limit [Å]	3.000	3.000
Number of reflections	12275
<I/σ(I)>		9
Completeness [%]	100.0	100
Redundancy		19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	20% PEG3000, 0.2M NaCl, and HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K