4L60
Structure of C81R Mutant PCNA Protein Defective in Mismatch Repair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-03 |
Detector | NOIR-1 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 3 |
Unit cell lengths | 121.822, 121.822, 121.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.500 - 3.003 |
R-factor | 0.2011 |
Rwork | 0.198 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.573 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.500 | 4.754 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 12275 | |
<I/σ(I)> | 9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 20% PEG3000, 0.2M NaCl, and HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |