4L5T
Crystal structure of the tetrameric p202 HIN2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.892, 71.860, 95.465 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 46.388 - 3.405 |
| R-factor | 0.2122 |
| Rwork | 0.206 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB IDs 2OQ0 and 3B6Y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.119 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 7.320 | 3.400 |
| Rmerge | 0.210 | 0.116 | 0.872 |
| <I/σ(I)> | 4.2 | ||
| Completeness [%] | 98.6 | 98 | 98.6 |
| Redundancy | 6.7 | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 22-25% PEG 3350, 0.1 M Tris pH 8.5 and 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
