4L5S
p202 HIN1 in complex with 12-mer dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.075 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 111.309, 156.118, 117.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.970 - 2.940 |
R-factor | 0.207 |
Rwork | 0.204 |
R-free | 0.25500 |
Starting model (for MR) | 4l5q |
RMSD bond length | 0.008 |
RMSD bond angle | 1.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.970 |
High resolution limit [Å] | 2.943 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 2.2 M ammonium sulfate, 0.1 M sodium cacodylate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |