4L5Q

Crystal structure of p202 HIN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9788
Spacegroup name	P 21 21 2
Unit cell lengths	71.544, 76.161, 45.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.838 - 2.230
R-factor	0.199
Rwork	0.197
R-free	0.23990
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.049
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.230
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	23-25% PEG 3350, 0.1 M Bis-Tris pH 5.6-5.8 and 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K