4L5O
Crystal structure of 26 kDa GST D26H mutant of Clonorchis sinensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.370, 98.370, 178.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.570 - 2.090 |
| R-factor | 0.20409 |
| Rwork | 0.202 |
| R-free | 0.24818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.768 |
| Data reduction software | DENZO |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 85.191 | 59.500 | 2.200 |
| High resolution limit [Å] | 2.089 | 6.610 | 2.090 |
| Rmerge | 0.019 | 0.019 | |
| Number of reflections | 60028 | ||
| <I/σ(I)> | 7.2 | 19.8 | 0.4 |
| Completeness [%] | 100.0 | 99.8 | 99.9 |
| Redundancy | 5.9 | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH8.5, 5mM Zinc sulfate, 2.0M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
