4L5I
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and its two different analogs reveal distinct mechanisms for ligand recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97940 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 176.744, 80.535, 103.635 |
Unit cell angles | 90.00, 112.18, 90.00 |
Refinement procedure
Resolution | 47.980 - 3.210 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.980 | 3.310 |
High resolution limit [Å] | 3.210 | 3.200 |
Number of reflections | 19846 | |
Completeness [%] | 79.1 | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M HEPES sodium, 17% isopropanol, 2% PEG3350, 2% Tacsimate, pH 7.0, VAPOR DIFFUSION, temperature 293K |