4L56
tRNA guanine transglycosylase H333D mutant apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.85507 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.049, 65.198, 69.547 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 29.391 - 1.700 |
| R-factor | 0.1614 |
| Rwork | 0.160 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pud |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.406 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 46368 | |
| <I/σ(I)> | 18 | 2.3 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris 10% DMSO 7% PEG 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
