4L4W
Structure of EspG3 chaperone from the type VII (ESX-3) secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.080, 123.690, 180.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.213 - 2.036 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5sxl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.213 | 2.090 |
| High resolution limit [Å] | 2.036 | 2.036 |
| Rmerge | 0.087 | 0.809 |
| Number of reflections | 33548 | |
| <I/σ(I)> | 12.15 | 1.55 |
| Completeness [%] | 97.6 | 79.5 |
| Redundancy | 5.5 | 3.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris-HCl, pH 8.5, 20% PEG6000, 1.0 M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
