4L4Q
Methionine Adenosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.839, 57.792, 236.434 |
| Unit cell angles | 90.00, 103.95, 90.00 |
Refinement procedure
| Resolution | 46.370 - 2.000 |
| R-factor | 0.18966 |
| Rwork | 0.187 |
| R-free | 0.24463 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.941 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.101 |
| Number of reflections | 119397 |
| <I/σ(I)> | 9.17 |
| Completeness [%] | 99.2 |
| Redundancy | 4.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM HEPES/NaOH pH 7.5, 35% pentaerythritol, propoxylate, 3% (w/v) sucrose, 0.2 M potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
