4L4H
Crystal structure of mouse Ryanodine Receptor isoform 2 (RyR2) 1-547
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 78.040, 78.040, 248.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.710 - 2.000 |
| R-factor | 0.2218 |
| Rwork | 0.220 |
| R-free | 0.25108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xoa |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.809 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 8.940 | 2.050 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.091 | 1.732 | |
| Number of reflections | 52836 | ||
| <I/σ(I)> | 1.99 | ||
| Completeness [%] | 99.6 | 83.3 | 99.9 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.15 | 294 | 6% PEG3350, 0.1 M Tris, pH 8.15, 0.8% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
