4L46
Crystal structures of human p70S6K1-WT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-12 |
| Detector | CCD ADSC unsupported-q315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.139, 81.139, 111.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.010 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.916 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.092 | 0.053 | 0.549 |
| Number of reflections | 8815 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 7.1 | 6.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.8 | 289 | 2.2M ammonium sulfate, 0.01M magnesium chloride, 0.1M HEPES, pH 6.8, hanging drop, temperature 289K |
