4L3R
Crystal structure of a DUF4847 family protein (BACEGG_01241) from Bacteroides eggerthii DSM 20697 at 2.23 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97949,0.97886 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.892, 59.174, 102.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.383 - 2.230 |
R-factor | 0.1999 |
Rwork | 0.198 |
R-free | 0.23230 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.383 | 29.383 | 2.310 |
High resolution limit [Å] | 2.230 | 4.780 | 2.230 |
Rmerge | 0.028 | 0.014 | 0.532 |
Number of reflections | 17344 | 3198 | 3103 |
<I/σ(I)> | 15.48 | 42.9 | 1.4 |
Completeness [%] | 95.0 | 94.6 | 93.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M citric acid pH 5, 20% polyethylene glycol 6000, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |