4L3N
Crystal structure of the receptor-binding domain from newly emerged Middle East respiratory syndrome coronavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.361, 108.065, 124.287 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.552 - 2.130 |
R-factor | 0.14707 |
Rwork | 0.144 |
R-free | 0.20602 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.335 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.552 | |
High resolution limit [Å] | 2.130 | 2.130 |
Number of reflections | 34770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 27% v/v PEG3350, 0.2 M magnesium chloride, 0.1 M Bis-Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |