4L3I
Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.205, 210.782, 112.294 |
Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
Resolution | 29.734 - 3.600 |
R-factor | 0.2617 |
Rwork | 0.257 |
R-free | 0.29990 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.762 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol ((phenix)) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.680 |
High resolution limit [Å] | 3.550 | 3.550 |
Number of reflections | 31794 | |
Completeness [%] | 96.2 | 83.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | Concentration: 5-10 mg/ml Protein buffer: 80 mM PIPES (pH 6.8), 1 mM MgCl2, 1 mM EGTA, 150 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 294K |