4L37
SP2-SP3 - a complex of two storage proteins from Bombyx mori hemolymph
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 192.750, 192.750, 180.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.000 - 2.900 |
| R-factor | 0.16726 |
| Rwork | 0.166 |
| R-free | 0.22267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.108 | 0.639 |
| Number of reflections | 44232 | |
| <I/σ(I)> | 23.2 | 5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.2 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.0 M ammonium sulfate 0.02 M sodium thiocyanate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
