4L37
SP2-SP3 - a complex of two storage proteins from Bombyx mori hemolymph
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 192.750, 192.750, 180.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.000 - 2.900 |
R-factor | 0.16726 |
Rwork | 0.166 |
R-free | 0.22267 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gwj |
RMSD bond length | 0.013 |
RMSD bond angle | 1.666 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.108 | 0.639 |
Number of reflections | 44232 | |
<I/σ(I)> | 23.2 | 5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.2 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.0 M ammonium sulfate 0.02 M sodium thiocyanate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |