4L2J
Crystal Structure of Osmotin, an antifungal laticifer protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.6080 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 95.590, 95.590, 79.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.101 - 1.610 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.175 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.101 | 1.900 |
| High resolution limit [Å] | 1.610 | 1.800 |
| Number of reflections | 25540 | |
| Completeness [%] | 87.9 | 83.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M HEPES buffer , 35% MPD, 0.7 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.5 |
