4L2I
Electron transferring flavoprotein of Acidaminococcus fermentans: Towards a mechanism of flavin-based electron bifurcation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.520, 84.790, 106.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.070 - 1.450 |
| R-factor | 0.15296 |
| Rwork | 0.150 |
| R-free | 0.20772 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.363 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.450 |
| Number of reflections | 124710 |
| Completeness [%] | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 17% PEG 3350, 0.1M Bis-Tris-Prop pH 7.5, 0.2M Sodium Formiat, 0.1M NaCl, 5mM NaDH, 1mM FAD, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
