4L2A
X-ray structure of the C57R mutant of the iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Collection date | 2007-10-10 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.622, 103.736, 50.244 |
Unit cell angles | 90.00, 108.29, 90.00 |
Refinement procedure
Resolution | 28.000 - 2.060 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.130 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.074 | 0.235 |
Number of reflections | 27167 | |
<I/σ(I)> | 13 | 4 |
Completeness [%] | 98.8 | 90.4 |
Redundancy | 4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | PEG 8K 23%, ammonium sulphate 0.2 M, sodium cacodilate 0.1 M, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |