4L1Z
Crystal structure of Cimex nitrophorin F64V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.294, 41.895, 65.773 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 35.290 - 1.650 |
| R-factor | 0.20531 |
| Rwork | 0.203 |
| R-free | 0.25603 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ntf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.965 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | REFMAC (5.7.003) |
| Refinement software | REFMAC (5.7.003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.120 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.063 | 0.299 |
| Number of reflections | 32298 | |
| <I/σ(I)> | 11.8 | 3.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.15 | 5.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
