4L1N

Crystal structure of a putative conserved lipoprotein (NT01CX_1156) from Clostridium novyi NT at 2.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-31
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	146.870, 146.870, 125.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.915 - 2.700
R-factor	0.1978
Rwork	0.198
R-free	0.20060
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.915		2.770
High resolution limit [Å]	2.700	12.080	2.700
Rmerge	0.100	0.011	0.011
Number of reflections	14440	180	1073
<I/σ(I)>	11.41	28.4	1.8
Completeness [%]	99.6	97.3	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.20000M lithium sulfate, 30.00000% polyethylene glycol 400, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K