4L1F
Electron transferring flavoprotein of Acidaminococcus fermentans: Towards a mechanism of flavin-based electron bifurcation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 109.100, 141.400, 64.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.130 - 1.790 |
R-factor | 0.15466 |
Rwork | 0.153 |
R-free | 0.18298 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 2.132 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.130 |
High resolution limit [Å] | 1.700 |
Number of reflections | 93076 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 25% PEG 1500, 10% (V/V) SPG Buffer, 25% Propandiol, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |