4L1F
Electron transferring flavoprotein of Acidaminococcus fermentans: Towards a mechanism of flavin-based electron bifurcation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.100, 141.400, 64.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.130 - 1.790 |
| R-factor | 0.15466 |
| Rwork | 0.153 |
| R-free | 0.18298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.132 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.130 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 93076 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 25% PEG 1500, 10% (V/V) SPG Buffer, 25% Propandiol, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
