4L1E
Crystal structure of C-Phycocyanin from Leptolyngbya sp. N62DM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.467, 107.107, 111.089 |
| Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
| Resolution | 24.978 - 2.610 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uum |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.928 |
| Data reduction software | AUTOMAR |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 61840 |
| Completeness [%] | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | PEG 2000, MgCl2.6H2O,Tris-Cl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
