4L1C

Crystal structure of Dimerized N-terminal Domain of MinC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.667, 53.983, 64.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.750 - 2.280
Rwork	0.229
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.418
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.750	2.340
High resolution limit [Å]	2.280	2.300
Rmerge	0.065	0.415
Number of reflections	8474
<I/σ(I)>	13.8	4.6
Completeness [%]	99.8	98.8
Redundancy	11.3	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	1.4 M Sodium citrate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K