4L16

Crystal structure of FIGL-1 AAA domain in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-26
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 65
Unit cell lengths	113.539, 113.539, 58.101
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.164 - 2.800
R-factor	0.2015
Rwork	0.200
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.309
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.200	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	10055
Completeness [%]	96.4	84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris, 0.5 M Sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K