4L15

Crystal structure of FIGL-1 AAA domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	108.295, 108.295, 54.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.541 - 2.600
R-factor	0.1884
Rwork	0.186
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.541	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	11481
Completeness [%]	97.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M Tris-HCl, 0.5 M sodium malonate, 0.3% PEG 10,000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K