4L11

Structure of the C-linker/CNBHD of agERG channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Collection date	2010-10-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	51.400, 51.400, 162.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.351 - 2.550
R-factor	0.2209
Rwork	0.219
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ukn
RMSD bond length	0.009
RMSD bond angle	1.333
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.510	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.082
Number of reflections	8662
<I/σ(I)>	17	3.3
Completeness [%]	100.0	100
Redundancy	12.1	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	9 mM Urea, 18.2% (w/v) PEG 3350, 7.3 % (v/v) Tacsimate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K