4L0W

Plasmodium yoelii Prx1a modified at the N-terminus forms an artifactual octamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-12-01
Detector	RIGAKU RAXIS V
Wavelength(s)	1.54178
Spacegroup name	P 4 2 2
Unit cell lengths	105.076, 105.076, 41.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 2.290
R-factor	0.1979
Rwork	0.196
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h01
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.153	0.471
Number of reflections	10337
Completeness [%]	94.6	91.5
Redundancy	13.9	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	291.15	1.5 ul of 4.3 mg/mL protein solution + 1.5 ul reservoir solution 1.6 M AMMONIUM SULFATE, 100 mM HEPES pH 6.8, 200 mM NaAc, 20 MM NaBr, 5% ETHYLENE GLYCOL, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K