4L0U
Crystal structure of Plasmodium vivax Prx1a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.550, 149.590, 131.910 |
| Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
| Resolution | 40.250 - 2.500 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h66 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.560 | 2.530 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.115 | 0.488 |
| Number of reflections | 86441 | |
| <I/σ(I)> | 10.8 | 1.3 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 300 | 1.5 uL of 8 mg/mL protein in 10 mM HEPES pH 7.5 500 mM NaCl + 1.5 uL reservoir solution 5% PEG 4000, 50 mM NaAc, 100 mM NaAc, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
