4L0K
Crystal structure of a type II restriction endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.397, 119.063, 82.111 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 35.724 - 2.328 |
| R-factor | 0.1945 |
| Rwork | 0.192 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.940 | 2.330 |
| Number of reflections | 47240 | ||
| Completeness [%] | 98.3 | 99 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 20% 2-propanol, 0.1M Tris pH 8.0, 5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
