4L0J

Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.8726
Spacegroup name	P 43 21 2
Unit cell lengths	109.173, 109.173, 56.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.820 - 1.850
R-factor	0.1807
Rwork	0.178
R-free	0.22789
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	2.253
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.820	2.170	1.910
High resolution limit [Å]	1.850	2.110	1.850
Number of reflections	29933
Completeness [%]	100.0	100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	0.1 M Bis-Tris propane, pH 6.5, 0.2 M lithium sulfate, 25% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 290K