4L0C
Crystal structure of the N-Fopmylmaleamic acid deformylase Nfo(S94A) from Pseudomonas putida S16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.412, 87.268, 130.879 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 44.570 - 1.650 |
| R-factor | 0.18635 |
| Rwork | 0.185 |
| R-free | 0.21126 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.570 | |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 239055 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 25% PEG8000, 0.3M NaBr, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
