4L0C
Crystal structure of the N-Fopmylmaleamic acid deformylase Nfo(S94A) from Pseudomonas putida S16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.412, 87.268, 130.879 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 44.570 - 1.650 |
R-factor | 0.18635 |
Rwork | 0.185 |
R-free | 0.21126 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.284 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.570 | |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 239055 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 25% PEG8000, 0.3M NaBr, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |