4KZZ

Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 31 2 1
Unit cell lengths	297.750, 297.750, 485.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	77.529 - 7.030
R-factor	0.3454
Rwork	0.345
R-free	0.35920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3J3A AND 3J3D
RMSD bond length	0.026
RMSD bond angle	2.743
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	80.000
High resolution limit [Å]	7.030
Number of reflections	38696
Completeness [%]	99.8
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	292	0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K