4KZZ
Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 297.750, 297.750, 485.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.529 - 7.030 |
R-factor | 0.3454 |
Rwork | 0.345 |
R-free | 0.35920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3J3A AND 3J3D |
RMSD bond length | 0.026 |
RMSD bond angle | 2.743 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.000 |
High resolution limit [Å] | 7.030 |
Number of reflections | 38696 |
Completeness [%] | 99.8 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 292 | 0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K |