4KYZ
Three-dimensional structure of triclinic form of de novo design insertion domain, Northeast Structural Genomics Consortium (NESG) Target OR327
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.819, 55.402, 78.258 |
| Unit cell angles | 82.03, 80.34, 90.03 |
Refinement procedure
| Resolution | 29.457 - 2.492 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ky3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.077 |
| Refinement software | PHENIX (dev_1269) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.492 |
| Rmerge | 0.082 |
| Number of reflections | 23740 |
| <I/σ(I)> | 15 |
| Completeness [%] | 95.1 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:0.1M NH4H2PO4 0.1M Tris PEG8K 20% , microbatch under oil, temperature 277K |
