4KXR
Structure of the Mycobacterium tuberculosis type VII secretion system chaperone EspG5 in complex with PE25-PPE41 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 139.100, 139.100, 171.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.241 - 2.600 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g38 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.241 | 2.670 | |
| High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
| Rmerge | 0.120 | 0.015 | 0.015 |
| Number of reflections | 30602 | 409 | 2208 |
| <I/σ(I)> | 15.81 | 41.16 | 1.8 |
| Completeness [%] | 99.9 | 97.1 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 295 | 0.1 M Tris-HCl, pH 8.6, 8% PEG8000, 0.2 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
