4KXP

Crystal Structure of AMP complexes of Porcine Liver Fructose-1,6-bisphosphatase Mutant I10D in T-state

Replaces:  2F3H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	59.544, 166.382, 78.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.660 - 2.700
R-factor	0.2068
Rwork	0.204
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.551
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.090	36.090	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.091	0.051	0.269
Total number of observations		7178	4220
Number of reflections	19025
<I/σ(I)>	8.2	16.2	3
Completeness [%]	85.3	85.9	84.1
Redundancy	2.38	3.44	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	PEG 3350, t-butyl alcohol, 2-methyl-2,4-pentanediol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K