4KX5
Cytosolic 5'-nucleotidase III complexed with cytidine 5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 46.846, 46.846, 285.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.290 - 1.900 |
| R-factor | 0.1567 |
| Rwork | 0.154 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fe3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.677 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.070 | 0.540 | |
| Number of reflections | 26476 | ||
| <I/σ(I)> | 39.1 | ||
| Completeness [%] | 99.2 | 97.5 | 98.7 |
| Redundancy | 10.5 | 8.9 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 20% PEG 3350, 0.10 M HEPES pH 8.0, 0.050 M Ammonium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
