4KWX
Linear structure of the Holliday junction sequence (TCGGCGCCGA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97630 |
Spacegroup name | P 1 |
Unit cell lengths | 34.410, 34.490, 34.690 |
Unit cell angles | 101.28, 103.21, 103.81 |
Refinement procedure
Resolution | 32.299 - 1.300 |
R-factor | 0.1737 |
Rwork | 0.173 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.263 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.570 | 32.570 | 1.340 |
High resolution limit [Å] | 1.300 | 5.370 | 1.300 |
Rmerge | 0.030 | 0.030 | 0.280 |
Number of reflections | 31546 | ||
<I/σ(I)> | 18.61 | 2.31 | |
Completeness [%] | 87.9 | 93.99 | 87.88 |
Redundancy | 1.77 | 1.83 | 1.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 0.75-1.5 mM DNA, 40-60% MPD, magnesium chloride, potassium chloride, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |