4KWO
tRNA guanine transglycosylase (TGT) in complex with Furanoside-Based lin-Benzoguanine 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-23 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.160, 65.047, 71.411 |
Unit cell angles | 90.00, 94.02, 90.00 |
Refinement procedure
Resolution | 26.000 - 1.320 |
R-factor | 0.1473 |
Rwork | 0.146 |
R-free | 0.16340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDN ENTRY 1PUD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.072 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.340 |
High resolution limit [Å] | 1.320 | 1.320 |
Number of reflections | 90020 | |
<I/σ(I)> | 28 | 3 |
Completeness [%] | 98.3 | 96.9 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |