4KWE
GDP-bound, double-stranded, curved FtsZ protofilament structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 200 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 132.509, 132.509, 321.549 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.950 - 2.910 |
R-factor | 0.24204 |
Rwork | 0.241 |
R-free | 0.26749 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.981 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.134 | 0.634 |
Number of reflections | 37414 | |
<I/σ(I)> | 65.7 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 21.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 3M Na formate, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |