4KWC

Structure of the plantazolicin methyltransferase BpumL in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 2
Unit cell lengths	67.222, 77.875, 44.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.879 - 1.994
R-factor	0.1912
Rwork	0.188
R-free	0.25640
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.994	1.994
Number of reflections	15809
Completeness [%]	94.8	68.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	278	25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K