4KW2
Crystal structure of a Putative uncharacterized protein (BDI_1873) from Parabacteroides distasonis ATCC 8503 at 2.32 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8434,0.9787,0.96911 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 200.095, 200.095, 200.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.881 - 2.320 |
R-factor | 0.1847 |
Rwork | 0.183 |
R-free | 0.21960 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.977 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.881 | 28.881 | 2.380 |
High resolution limit [Å] | 2.320 | 10.380 | 2.320 |
Rmerge | 0.026 | 0.026 | |
Total number of observations | 6924 | 40026 | |
Number of reflections | 15409 | ||
<I/σ(I)> | 13.7 | 8.5 | 0.3 |
Completeness [%] | 100.0 | 95.6 | 100 |
Redundancy | 35.8 | 31.8 | 36.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.19 | 293 | 1.48M ammonium sulfate, 0.1M phosphate-citrate pH 4.19, Additive 0.005 M Myo-Inositol, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |