4KW0
Structure of Dickerson-Drew Dodecamer with 2'-MeSe-ara-G modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.780, 39.893, 65.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.490 |
| R-factor | 0.20473 |
| Rwork | 0.203 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bna |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 10484 | |
| <I/σ(I)> | 14.75 | 2.93 |
| Completeness [%] | 0.9 | 0.892 |
| Redundancy | 5.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% v/v MPD, 40 mM Sodium Cacodylate, 12 mM Spermine tetra-HCl, 80 mM Sodium Chloride, 20 mM Magnesium Chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
