4KVT
Crystal structure of a 6-helix coiled coil CC-Hex-L24C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 65 |
Detector technology | PIXEL |
Collection date | 2012-10-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 54.430, 54.430, 147.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.098 - 1.600 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.20840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.912 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.160 |
High resolution limit [Å] | 1.600 |
Number of reflections | 29509 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1 M Tris, 0.1 M potassium chloride with 15 % w/v PEG 2000 MME, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |