4KUN
Crystal structure of Legionella pneumophila Lpp1115 / KaiB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2828144 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.830, 46.050, 45.079 |
| Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
| Resolution | 27.379 - 1.950 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.24670 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.801 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.910 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.061 | 0.484 |
| Number of reflections | 11748 | |
| <I/σ(I)> | 21.7 | 5.1 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M sodium acetate, 0.1 M bis-Tris pH 6.5, 35% w/v PEG 8K, 1 mM zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
