4KUL
Crystal structure of N-terminal acetylated yeast Sir3 BAH domain V83P mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.227, 43.453, 53.636 |
| Unit cell angles | 90.00, 97.43, 90.00 |
Refinement procedure
| Resolution | 46.720 - 2.620 |
| R-factor | 0.2208 |
| Rwork | 0.217 |
| R-free | 0.27742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fvu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.095 | 0.393 |
| Number of reflections | 6570 | |
| <I/σ(I)> | 12.7 | 3.6 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 14% 2-propanol, 0.2M CaCl, 0.1M sodium acetate(pH4.6) , VAPOR DIFFUSION, HANGING DROP, temperature 289K |
