4KUI
Crystal structure of N-terminal acetylated yeast Sir3 BAH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9760 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.599, 44.768, 60.487 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 35.013 - 1.850 |
| R-factor | 0.1727 |
| Rwork | 0.171 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fvu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.068 | 0.549 |
| Number of reflections | 20961 | |
| <I/σ(I)> | 19.1 | 2.6 |
| Completeness [%] | 98.5 | 98.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 18% 2-propanol, 0.2M CaCl, 0.1M sodium acetate (pH4.6), VAPOR DIFFUSION, HANGING DROP, temperature 289K |
