4KU4
Crystal Structure of a Ras-like Protein from Cryphonectria parasitica in Complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 36.795, 52.595, 53.234 |
Unit cell angles | 114.62, 93.01, 90.05 |
Refinement procedure
Resolution | 44.500 - 1.600 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.22140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ce2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.510 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.075 | 0.313 |
Number of reflections | 42642 | |
<I/σ(I)> | 11.07 | 2.9 |
Completeness [%] | 88.4 | 62.8 |
Redundancy | 3.5 | 2.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 5 mM GDP, 0.2 M Magnesium Format, 0.1 M HEPES, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |