4KU0
Enterobacteria phage T4 gp5.4 PAAR repeat protein in complex with T4 gp5 beta-helix fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.302, 49.327, 84.057 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 46.000 - 1.150 |
| R-factor | 0.1265 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jj2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.220 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.053 | |
| Number of reflections | 124490 | |
| <I/σ(I)> | 12.12 | 2.27 |
| Completeness [%] | 96.5 | 81.3 |
| Redundancy | 6.13 | 4.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 23-25% PEG 3350, 100mM Tris pH=8.5, 40-100mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
