4KTZ
Lactococcus phage 67 RuvC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.710, 66.970, 71.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.110 - 1.900 |
| R-factor | 0.20451 |
| Rwork | 0.202 |
| R-free | 0.25374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ktw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.243 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 29210 | |
| <I/σ(I)> | 8.1 | 2.4 |
| Completeness [%] | 94.5 | 88.3 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.4 M MgCl2, 25% w/v PEG2000, 0.1M HEPES pH7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
