4KTW

Lactococcus phage 67 RuvC - apo form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8856, 0.9785, 0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	63.060, 66.900, 73.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.450 - 1.680
R-factor	0.19912
Rwork	0.197
R-free	0.23714
Structure solution method	MAD
RMSD bond length	0.022
RMSD bond angle	1.884
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.500	1.800
High resolution limit [Å]	1.680	1.700
Number of reflections	35135
Completeness [%]	100.0	100
Redundancy		7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	20% w/v PEG3350, 0.1 M KCl, 0.1M Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K